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J Biomater Appl. 2009 Sep;24(3):275-87. doi: 10.1177/0885328208096534. Epub 2008 Nov 5.

Pluronic F-127 as a cell carrier for bone tissue engineering.

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Orthopaedic Research Laboratory, Department of Orthopaedics, Centre hospitalier Sacré-Coeur, Montréal, Québec, Canada.


The objective of this study is to report the effect of Pluronic F-127 on osteoblast viability and phenotype maintenance in vitro. MG-63 cells are suspended in Pluronic F-127, and MTT assay, alkaline phosphatase activity, prostaglandin E(2) production, collagen-I, and cyclo-oxygenase-2 expression are assessed up to 6 days. Pluronic F-127 leads to a significant decrease in osteoblast viability throughout the 6-day experiment, without altering osteoblast phenotype. The addition of platelet-rich plasma to the polymer/cell construct leads to increased cell survival. When supplemented with bioactive factors, Pluronic F-127 could potentially be used as a cell carrier in bone tissue engineering.

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