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J Phys Chem B. 2008 Nov 27;112(47):14678-81. doi: 10.1021/jp807784j.

Construction of asymmetric cell-sized lipid vesicles from lipid-coated water-in-oil microdroplets.

Abstract

We present a simple, rapid, and robust method for preparing asymmetric cell-sized lipid bilayer vesicles using water-in-oil (W/O) microdroplets transferred through an oil-water interface. The efficiency for producing cell-sized model membranes is elucidated in relation to the vesicular size and the weight of contained water-soluble molecules. We demonstrate the biological asymmetric nature and the formation of lipid raft microdomain structures using fluorescence microscopy.

PMID:
18983183
DOI:
10.1021/jp807784j
[Indexed for MEDLINE]
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