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J Vis Exp. 2007;(5):215. doi: 10.3791/215. Epub 2007 Jul 4.

Injection of dsRNA into female A. aegypti mosquitos.

Author information

1
Department of Molecular Biology and Biochemistry, University of California, Irvine, USA.

Abstract

Reverse genetic approaches have proven extremely useful for determining which genes underly resistance to vector pathogens in mosquitoes. This video protocol illustrates a method used by the James lab to inject dsRNA into female A. aegypti mosquitoes, which harbor the dengue virus. The technique for calibrating injection needles, manipulating the injection setup, and injecting dsRNA into the thorax is illustrated.

PMID:
18979015
PMCID:
PMC2557097
DOI:
10.3791/215
[Indexed for MEDLINE]
Free PMC Article

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