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J Biol Chem. 2008 Dec 26;283(52):36132-9. doi: 10.1074/jbc.M808152200. Epub 2008 Oct 31.

FANCJ is a structure-specific DNA helicase associated with the maintenance of genomic G/C tracts.

Author information

1
MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK.

Abstract

Fanconi anemia (FA) is a heritable human cancer-susceptibility disorder, delineating a genetically heterogenous pathway for the repair of replication-blocking lesions such as interstrand DNA cross-links. Here we demonstrate that one component of this pathway, FANCJ, is a structure-specific DNA helicase that dissociates guanine quadruplex DNA (G4 DNA) in vitro. Moreover, in contrast with previously identified G4 DNA helicases, such as the Bloom's helicase (BLM), FANCJ unwinds G4 substrates with 5'-3' polarity. In the FA-J human patient cell line EUFA0030 the loss of FANCJ G4 unwinding function correlates with the accumulation of large genomic deletions in the vicinity of sequences, which match the G4 DNA signature. Together these findings support a role for FANCJ in the maintenance of potentially unstable genomic G/C tracts during replication.

PMID:
18978354
PMCID:
PMC2662291
DOI:
10.1074/jbc.M808152200
[Indexed for MEDLINE]
Free PMC Article

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