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Fertil Steril. 2009 Sep;92(3):1141-6. doi: 10.1016/j.fertnstert.2008.07.1751. Epub 2008 Oct 31.

Inhibition of human sperm function and mouse fertilization in vitro by an antibody against cation channel of sperm 1: the contraceptive potential of its transmembrane domains and pore region.

Author information

1
Family Planning Research Institute, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China.

Abstract

OBJECTIVE:

To explore the contraceptive potential of the CatSper1 transmembrane domains and pore region in vitro.

DESIGN:

In vitro study with human sperm and mouse fertilization.

SETTING:

Andrology laboratory of an academic research center.

PATIENT(S) AND ANIMAL(S):

Normozoospermia and viripotent BALB/c mice.

INTERVENTION(S):

The specific binding of an anti-CatSper1 IgG antibody (H-300) to CatSper1 was confirmed by Western blot and immunofluorescence. Sperm from humans and mice were incubated with H-300.

MAIN OUTCOME MEASURE(S):

The effects of H-300 on human sperm progressive motility, abnormal acrosome, hyperactivated motility, and mouse in vitro fertilization rates were analyzed.

RESULT(S):

A significant decline in sperm progressive motility was observed after 1, 2, and 4 hours of incubation with H-300; the change was mainly ascribed to the decline of fast progressive motility. Significant inhibition of the hyperactivated motility was observed after 5 hours of incubation with H-300. The incubation of mouse sperm with H-300 before insemination reduced the in vitro fertilization rate to 28% of control levels (72% inhibition).

CONCLUSION(S):

CatSper1 may be a potential target for immunocontraception, and the antibody may be a tool to study the function of ion channels in sperm in which relatively fewer methods can be applied.

[Indexed for MEDLINE]

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