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Nat Methods. 2008 Nov;5(11):927-33. doi: 10.1038/nmeth.1265.

Native mass spectrometry: a bridge between interactomics and structural biology.

Author information

1
Biomolecular Mass Spectrometry and Proteomics Group, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands. a.j.r.heck@uu.nl

Abstract

Native mass spectrometry is an emerging technology that allows the topological investigation of intact protein complexes with high sensitivity and a theoretically unrestricted mass range. This unique tool provides complementary information to established technologies in structural biology, and also provides a link to high-throughput interactomics studies, which do not generate information on exact protein complex-composition, structure or dynamics. Here I review the current state of native mass spectrometry technology and discuss several important biological applications. I also describe current experimental challenges in native mass spectrometry, encouraging readers to contribute to solutions.

PMID:
18974734
DOI:
10.1038/nmeth.1265
[Indexed for MEDLINE]

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