Format

Send to

Choose Destination
See comment in PubMed Commons below
J Virol. 2009 Jan;83(2):633-9. doi: 10.1128/JVI.01766-08. Epub 2008 Oct 29.

Temporal control of message stability in the life cycle of double-stranded RNA bacteriophage phi8.

Author information

1
Department of Microbiology, the Public Health Research Institute, Newark, New Jersey 07103, USA.

Abstract

The cystoviruses have genomes of three double-stranded RNA segments. The genes of the L transcript are expressed early in infection, while those of M and S are expressed late. In all cystovirus groups but one, the quantity of the L transcript late in infection is lower than those of the other two because of transcriptional control. In bacteriophage Phi8 and its close relatives, transcription of L is not controlled; instead, the L transcript is turned over rapidly late in infection. The three messages are produced in approximately equal amounts early in infection, but the amount of L is less than 10% of the amounts of the others late in infection. The decay of the Phi8 L message depends upon the production of protein Hb, which is encoded in segment L. It also depends upon a target site within the H gene region. Phage mutants lacking either the Hb gene or the target region do not show the late control of L message quantity. In addition to having a role as a negative regulator, Hb functions to neutralize the activity of protein J, encoded by segment S, which causes the degradation of all viral transcripts.

PMID:
18971268
PMCID:
PMC2612379
DOI:
10.1128/JVI.01766-08
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Support Center