Effect of cetyltrimethylammonium bromide on the migration of polyaromatic hydrocarbons in capillary electrokinetic chromatography

G Kavran Belin; F B Erim; F O Gülaçar

doi:10.1016/j.talanta.2005.10.025

Effect of cetyltrimethylammonium bromide on the migration of polyaromatic hydrocarbons in capillary electrokinetic chromatography

Talanta. 2006 May 15;69(3):596-600. doi: 10.1016/j.talanta.2005.10.025. Epub 2005 Nov 28.

Authors

G Kavran Belin¹, F B Erim, F O Gülaçar

Affiliation

¹ Laboratory of Mass Spectrometry, University of Geneva, Sciences I, Boulevard d'Yvoy 16, CH-1211, Geneva 4, Switzerland; Technical University of Istanbul, Department of Chemistry, 34469 Maslak, Istanbul, Turkey.

PMID: 18970609
DOI: 10.1016/j.talanta.2005.10.025

Abstract

The separation of different ring numbered polyaromatic hydrocarbons (PAHs) was accomplished by using cetyltrimethylammonium bromide (CTAB) in capillary electrokinetic chromatography. In order to increase the solubilities and selectivities of PAHs, acetonitrile (ACN) was used as an organic modifier. Under the optimised conditions, 11 aromatic compounds were separated within 14.5min in a running electrolyte containing 10mM phosphate, 30mM CTAB, and 40% ACN at pH 6.0. The effects of CTAB and ACN concentrations, voltage and pH on the resolution were investigated. Reproducibilities of migration times range between 0.55 and 1.27 R.S.D.% and peak areas between 1.02 and 7.23 R.S.D.%. Limit of detections (LODs) range between 0.09 and 2.24mugml(-1). This new and fast separation method of PAHs was applied to cooked oil sample.