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Talanta. 2005 Jun 15;66(5):1219-24. doi: 10.1016/j.talanta.2005.01.026.

Amperometric detection of mono- and diphenols at Cerrena unicolor laccase-modified graphite electrode: correlation between sensitivity and substrate structure.

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  • 1Department of Biochemistry, Maria Curie-Sklodowska University, Sklodowska Square 3, 20-031 Lublin, Poland.

Abstract

Graphite electrode modified with laccase from Cerrena unicolor served as a biosensor for detection of 30 phenolic compounds with different structures. Some correlations of the sensor response to the structures of substrates are discussed. This biosensor responded to: (i) nanomolar concentrations of some of the selected phenolic compounds, e.g., 2,6-dimethoxyphenol, coniferyl alcohol, caffeic acid, DOPAC and hydroquinone, (ii) micromolar concentrations, e.g., ferulic acid, syringic acid, dopamine, 3,4-dihydroxybenzoic acid and dl-noradrenaline, and (iii) millimolar concentrations in the case of phenol and 4-hydroxybenzaldehyde. Among the ortho- or para-substituted phenols, the sensitivity of the C. unicolor laccase-modified electrode increased in the following order H, CH(3), OH, OCH(3) and NH(3)(+) but in the case of para-substituted phenols, the K(m)(app) values were lower. The sensitivity of the laccase electrode increased with an additional OH group in para-substituted phenols. In the case of the selected compounds, kinetic data from electrochemical flow injection system were compared with those obtained from experiments in solution.

PMID:
18970111
DOI:
10.1016/j.talanta.2005.01.026
[PubMed]
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