Influence of organized media on the absorption and fluorescence spectra of Auramine-O dye

A G Mwalupindi; A Rideau; R A Agbaria; I M Warner

doi:10.1016/0039-9140(93)e0033-a

Influence of organized media on the absorption and fluorescence spectra of Auramine-O dye

Talanta. 1994 Apr;41(4):599-609. doi: 10.1016/0039-9140(93)e0033-a.

Authors

A G Mwalupindi¹, A Rideau, R A Agbaria, I M Warner

Affiliation

¹ Department of Chemistry, Louisiana State University, Baton Rouge, LA 70803, U.S.A.

PMID: 18965970
DOI: 10.1016/0039-9140(93)e0033-a

Abstract

The spectral characteristics of the aqueous solutions of Auramine O dye in the presence of organized media have been examined. The absorbance and fluorescence of the dye are significantly enhanced in cyclodextrins and surfactants. The dye forms a predominant 1:1 complex with beta-CD, whereas a 1:2 complex is formed with alpha-CD. The dissociation constants of the complexes have been determined by use of a non-linear regression method. Premicellar solutions of the sodium dodecyl sulphate exert maximum influence on the fluorescence and absorbance spectra of the dye. The microviscosity in cyclodextrins and micelles of SDS and Brij-78 have been estimated from fluorescence measurements.