Format

Send to

Choose Destination
Genetics. 2009 Jan;181(1):335-40. doi: 10.1534/genetics.108.093104. Epub 2008 Oct 28.

Drosophila hold'em is required for a subset of meiotic crossovers and interacts with the dna repair endonuclease complex subunits MEI-9 and ERCC1.

Author information

1
Department of Genetics, Rutgers, the State University of New Jersey, Piscataway, New Jersey 08854-8020, USA.

Abstract

Three Drosophila proteins, ERCC1, MUS312, and MEI-9, function in a complex proposed to resolve double-Holliday-junction intermediates into crossovers during meiosis. We report here the characterization of hold'em (hdm), whose protein product belongs to a single-strand-DNA-binding superfamily of proteins. Mutations in hdm result in reduced meiotic crossover formation and sensitivity to the DNA-damaging agent methyl methanesulfonate. Furthermore, HDM physically interacts with both MEI-9 and ERCC1 in a yeast two-hybrid assay. We conclude that HDM, MEI-9, MUS312, and ERCC1 form a complex that resolves meiotic recombination intermediates into crossovers.

PMID:
18957705
PMCID:
PMC2621184
DOI:
10.1534/genetics.108.093104
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center