Format

Send to

Choose Destination
PLoS One. 2008;3(10):e3537. doi: 10.1371/journal.pone.0003537. Epub 2008 Oct 27.

Osteoclasts control osteoblast chemotaxis via PDGF-BB/PDGF receptor beta signaling.

Author information

1
Biotechnological Center, Dresden University of Technology, Dresden, Germany.

Abstract

BACKGROUND:

Bone remodeling relies on the tightly regulated interplay between bone forming osteoblasts and bone digesting osteoclasts. Several studies have now described the molecular mechanisms by which osteoblasts control osteoclastogenesis and bone degradation. It is currently unclear whether osteoclasts can influence bone rebuilding.

METHODOLOGY/PRINCIPAL FINDINGS:

Using in vitro cell systems, we show here that mature osteoclasts, but not their precursors, secrete chemotactic factors recognized by both mature osteoblasts and their precursors. Several growth factors whose expression is upregulated during osteoclastogenesis were identified by DNA microarrays as candidates mediating osteoblast chemotaxis. Our subsequent functional analyses demonstrate that mature osteoclasts, whose platelet-derived growth factor bb (PDGF-bb) expression is reduced by siRNAs, exhibit a reduced capability of attracting osteoblasts. Conversely, osteoblasts whose platelet-derived growth factor receptor beta (PDGFR-beta) expression is reduced by siRNAs exhibit a lower capability of responding to chemotactic factors secreted by osteoclasts.

CONCLUSIONS/SIGNIFICANCE:

We conclude that, in vitro mature osteoclasts control osteoblast chemotaxis via PDGF-bb/PDGFR-beta signaling. This may provide one key mechanism by which osteoclasts control bone formation in vivo.

PMID:
18953417
PMCID:
PMC2569415
DOI:
10.1371/journal.pone.0003537
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Public Library of Science Icon for PubMed Central
Loading ...
Support Center