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J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Nov 15;875(2):522-30. doi: 10.1016/j.jchromb.2008.10.011. Epub 2008 Oct 14.

A liquid chromatography/electrospray ionization mass spectrometry (LC-MS/MS) assay for the determination of irinotecan (CPT-11) and its two major metabolites in human liver microsomal incubations and human plasma samples.

Author information

1
Pharmacogenomics and Drug Development Group, Faculty of Pharmacy, The University of Sydney, NSW 2006, Australia. fabrizio@pharm.usyd.edu.au

Abstract

A sensitive, rapid LC-MS/MS assay has been developed and validated for the simultaneous quantification of CPT-11 and its two principal metabolites, 7-ethyl-10-hydroxycamptothecin (SN-38), and 7-ethyl-10-[4-N-(5-aminopentanoic acid)-1-piperidino]carbonyloxy-camptothecin (APC) in human liver microsomal fractions and plasma. The method was linear over the ranges of 1.56-100 ng/mL, 3.13-150 ng/mL, and 0.78-100 ng/mL for CPT-11, SN-38, and APC, respectively. The total run time was 7.0 min. This assay offers advantages in terms of expediency, recovery of analytes, and suitability for the analysis of CPT-11 and its metabolites in various biological fluids.

PMID:
18952506
DOI:
10.1016/j.jchromb.2008.10.011
[Indexed for MEDLINE]

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