Format

Send to

Choose Destination
Nutrition. 2009 Jan;25(1):40-4. doi: 10.1016/j.nut.2008.07.006. Epub 2008 Oct 26.

Inductions of histone H3 acetylation at lysine 9 on SGLT1 gene and its expression by feeding mice a high carbohydrate/fat ratio diet.

Author information

1
Laboratory of Nutritional Physiology, Graduate School of Nutritional and Environmental Sciences and Global COE, The University of Shizuoka, Shizuoka, Japan.

Abstract

OBJECTIVE:

We examined in this study whether histone H3 acetylations on jejunal NA(+)/glucose transport-1 (SGLT1) gene are associated with induction of its gene by feeding mice a high carbohydrate/fat ratio diet.

METHODS:

The chromatin immunoprecipitation (ChIP) assays using antibodies of acetylated histone H3 were performed in jejunum of mice fed a high carbohydrate/fat ratio diet or a low carbohydrate/fat ratio for 7 d.

RESULTS:

The acetylations of histone H3 of lysine 9/14 were highly detected on the SGLT1 gene and the levels were enhanced by feeding mice a high carbohydrate/fat ratio diet. Histone H3 acetylation at lysine 9 was enhanced by feeding a high carbohydrate/fat ratio diet, whereas that at lysine 14 was not enhanced significantly.

CONCLUSION:

These observations indicate that induction of SGLT1 gene expression by feeding a high carbohydrate/fat ratio diet is associated with acetylation of histone H3 at lysine 9 on the SGLT1 gene.

PMID:
18952408
DOI:
10.1016/j.nut.2008.07.006
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center