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Mol Immunol. 2009 Feb;46(5):1007-10. doi: 10.1016/j.molimm.2008.09.017. Epub 2008 Oct 25.

Nylon wool purification alters the activation of T cells.

Author information

1
Department of Microbiology, University of Alabama, Birmingham, Birmingham, AL 35294, USA.

Abstract

Purification of lymphocytes, particularly T cells, is commonly performed using nylon wool. This enrichment method selectively retains B cells and some myeloid cells allowing a significantly more pure T cell population to flow through a nylon wool column. T cells purified in this fashion are assumed to be unaltered and functionally naïve, however some studies have suggested aberrant in vitro T cell responses after nylon wool treatment. We found that nylon wool purification significantly altered T cell proliferation, expression of activation markers and production of cytokines. Our results suggest that nylon wool treatment modifies T cell activation responses and that caution should be used when choosing this purification method.

PMID:
18952296
PMCID:
PMC2671197
DOI:
10.1016/j.molimm.2008.09.017
[Indexed for MEDLINE]
Free PMC Article

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