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Methods. 2008 Dec;46(4):295-303. doi: 10.1016/j.ymeth.2008.10.003. Epub 2008 Oct 24.

Assessing mitochondrial morphology and dynamics using fluorescence wide-field microscopy and 3D image processing.

Author information

1
Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, 4000 Central Florida Blvd., Orlando, FL 32816.
2
The Burnham Institute for Medical Research, 10901 N. Torrey Pines Road, La Jolla, CA 92037.
3
University of Pennsylvania, 3451 Walnut Street, Philadelphia, PA.
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Contributed equally

Abstract

Mitochondrial morphology and length change during fission and fusion and mitochondrial movement varies dependent upon the cell type and the physiological conditions. Here, we describe fundamental wide-field fluorescence microscopy and 3D imaging techniques to assess mitochondrial shape, number and length in various cell types including cancer cell lines, motor neurons and astrocytes. Furthermore, we illustrate how to assess mitochondrial fission and fusion events by 3D time-lapse imaging and to calculate mitochondrial length and numbers as a function of time. These imaging methods provide useful tools to investigate mitochondrial dynamics in health, aging and disease.

PMID:
18952177
PMCID:
PMC3992922
DOI:
10.1016/j.ymeth.2008.10.003
[Indexed for MEDLINE]
Free PMC Article

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