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J Chromatogr A. 2008 Nov 28;1212(1-2):150-3. doi: 10.1016/j.chroma.2008.10.011. Epub 2008 Oct 10.

Hydroxyproline quantification for the estimation of collagen in tissue using multiple reaction monitoring mass spectrometry.

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Queensland Bioscience Precinct, St Lucia, QLD 4067, Australia.


Collagens are highly abundant mammalian proteins that contain a high content of hydroxylated amino acids such as hydroxyproline. We have exploited the high hydroxyproline content of collagen and developed a method for hydroxyproline quantification as a measure of collagen content in muscle samples. The novel method utilizes a highly selective and sensitive method of multiple reaction monitoring (MRM) by mass spectrometry. The analytical method is simple, rapid (5min), convenient (no derivatization), precise (<17% RSD), accurate (90-108%), sensitive (4.88nmol/L) and linear (R(2)>0.999) over three orders of magnitude (5-5000nmol/L).

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