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PLoS One. 2008;3(10):e3452. doi: 10.1371/journal.pone.0003452. Epub 2008 Oct 20.

A ligand peptide motif selected from a cancer patient is a receptor-interacting site within human interleukin-11.

Author information

1
The University of Texas MD Anderson Cancer Center, Houston, Texas, United States of America.

Abstract

Interleukin-11 (IL-11) is a pleiotropic cytokine approved by the FDA against chemotherapy-induced thrombocytopenia. From a combinatorial selection in a cancer patient, we isolated an IL-11-like peptide mapping to domain I of the IL-11 (sequence CGRRAGGSC). Although this motif has ligand attributes, it is not within the previously characterized interacting sites. Here we design and validate in-tandem binding assays, site-directed mutagenesis and NMR spectroscopy to show (i) the peptide mimics a receptor-binding site within IL-11, (ii) the binding of CGRRAGGSC to the IL-11R alpha is functionally relevant, (iii) Arg4 and Ser8 are the key residues mediating the interaction, and (iv) the IL-11-like motif induces cell proliferation through STAT3 activation. These structural and functional results uncover an as yet unrecognized receptor-binding site in human IL-11. Given that IL-11R alpha has been proposed as a target in human cancer, our results provide clues for the rational design of targeted drugs.

PMID:
18941632
PMCID:
PMC2565473
DOI:
10.1371/journal.pone.0003452
[Indexed for MEDLINE]
Free PMC Article

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