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PLoS One. 2008;3(10):e3498. doi: 10.1371/journal.pone.0003498. Epub 2008 Oct 22.

Kinetics of Wnt-driven beta-catenin stabilization revealed by quantitative and temporal imaging.

Author information

1
Department of Protein Engineering, Genentech Inc., South San Francisco, California, United States of America. hannoush.rami@gene.com

Abstract

The Wnt/beta-catenin signal transduction pathway regulates a broad range of developmental processes. Aberrant activation of the Wnt pathway leads to cancer and degenerative diseases. beta-catenin is a key signaling molecule that is frequently used as a direct monitor of Wnt pathway activation. This paper describes a multi-parametric method for quantitative analysis of cellular beta-catenin protein levels in a rapid and high-throughput manner. The assay offers temporally resolved detection of Wnt-stimulated accumulation of beta-catenin, simultaneously detecting cell number, and it sheds light onto the kinetics of posttranslational stabilization of beta-catenin.

PMID:
18941539
PMCID:
PMC2570213
DOI:
10.1371/journal.pone.0003498
[Indexed for MEDLINE]
Free PMC Article
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