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Oral Dis. 2008 Jul;14(5):428-34.

SHED repair critical-size calvarial defects in mice.

Author information

1
Department of Oral and Maxillofacial Surgery, College of Dentistry, Seoul National University, Seoul, Korea.

Erratum in

  • Oral Dis. 2009 May;15(4):302.

Abstract

OBJECTIVE:

Stem cells from human exfoliated deciduous teeth (SHED) are a population of highly proliferative postnatal stem cells capable of differentiating into odontoblasts, adipocytes, neural cells, and osteo-inductive cells. To examine whether SHED-mediated bone regeneration can be utilized for therapeutic purposes, we used SHED to repair critical-size calvarial defects in immunocompromised mice.

MATERIALS AND METHODS:

We generated calvarial defects and transplanted SHED with hydroxyapatite/tricalcium phosphate as a carrier into the defect areas.

RESULTS:

SHED were able to repair the defects with substantial bone formation. Interestingly, SHED-mediated osteogenesis failed to recruit hematopoietic marrow elements that are commonly seen in bone marrow mesenchymal stem cell-generated bone. Furthermore, SHED were found to co-express mesenchymal stem cell marker, CC9/MUC18/CD146, with an array of growth factor receptors such as transforming growth factor beta receptor I and II, fibroblast growth factor receptor I and III, and vascular endothelial growth factor receptor I, implying their comprehensive differentiation potential.

CONCLUSIONS:

Our data indicate that SHED, derived from neural crest cells, may select unique mechanisms to exert osteogenesis. SHED might be a suitable resource for orofacial bone regeneration.

PMID:
18938268
PMCID:
PMC2653202
DOI:
10.1111/j.1601-0825.2007.01396.x
[Indexed for MEDLINE]
Free PMC Article

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