Format

Send to

Choose Destination
J Chromatogr A. 2008 Nov 28;1212(1-2):106-13. doi: 10.1016/j.chroma.2008.10.006. Epub 2008 Oct 8.

Evaluation of a rapid method for the quantitative analysis of fatty acids in various matrices.

Author information

1
National Institute of Nutrition and Seafood Research, N-5817 Bergen, Norway. pedro.araujo@nifes.no

Abstract

A simplified method for quantitative analysis of fatty acids in various matrices by gas chromatography is proposed as an alternative to the conventional method and the variables of the protocol examined to optimize the processing conditions. The modified method involves direct methylation of fatty acids in homogenized samples with boron trihalide (BF(3) or BCl(3) in methanol) followed by extraction with hexane. The addition of hexane to the reaction mixture after the methylation process can enhance the efficiency of fatty acid methylation and is critical for those samples that contain high levels of triglycerides. A mechanism underlying this effect is proposed.

PMID:
18937951
PMCID:
PMC2593112
DOI:
10.1016/j.chroma.2008.10.006
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center