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Proteomics. 2008 Nov;8(22):4642-6. doi: 10.1002/pmic.200800216.

A novel solid phase- and chemical crosslinking-based technology for determining protein localization in biological supramolecules.

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1
Institute of Molecular Medicine, National Tsing Hua University, Hsinchu, Taiwan, ROC.

Abstract

A solid phase- and chemical crosslinking-based technology was developed for determining the depths at which various protein constituents reside in a supramolecule. The usefulness of this technology was verified by trials using a synthetic three-protein complex on glass coverslips. This technology was further applied to investigate the localization of seven major protein components in the postsynaptic density, a landmark supramolecule of the excitatory synapses in mammalian brains. The technology reported here will supplement the already powerful proteomic methodologies in studying the structure/function relationships of supramolecules.

PMID:
18937251
DOI:
10.1002/pmic.200800216
[Indexed for MEDLINE]
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