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Appl Environ Microbiol. 2008 Dec;74(24):7828-31. doi: 10.1128/AEM.01303-08. Epub 2008 Oct 17.

Cross-kingdom amplification using bacteria-specific primers: complications for studies of coral microbial ecology.

Author information

1
University of South Florida, College of Marine Science, 140 7th Ave. S., St. Petersburg, Florida 33701, USA.

Abstract

PCR amplification of pure bacterial DNA is vital to the study of bacterial interactions with corals. Commonly used Bacteria-specific primers 8F and 27F paired with the universal primer 1492R amplify both eukaryotic and prokaryotic rRNA genes. An alternative primer set, 63F/1542R, is suggested to resolve this problem.

PMID:
18931299
PMCID:
PMC2607152
DOI:
10.1128/AEM.01303-08
[Indexed for MEDLINE]
Free PMC Article

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