Abstract
A new lipase, LipEH166, isolated from an intertidal flat metagenome, showed no amino acid similarity to any known lipolytic enzyme except in the consensus region. This suggested that LipEH166 and its homologues belong to a new family of lipolytic enzymes. Partial characterization indicated that LipEH166 is a novel cold-adapted alkaline lipase.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / genetics*
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Bacterial Proteins / metabolism*
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Cloning, Molecular
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Cold Temperature
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics*
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DNA, Bacterial / isolation & purification*
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Enzyme Stability
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Hydrogen-Ion Concentration
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Lipase / chemistry
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Lipase / genetics*
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Lipase / metabolism*
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Molecular Sequence Data
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Phylogeny
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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Substrate Specificity
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Water Microbiology*
Substances
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Bacterial Proteins
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DNA, Bacterial
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Lipase