Format

Send to

Choose Destination
Biochem Biophys Res Commun. 2008 Dec 12;377(2):658-661. doi: 10.1016/j.bbrc.2008.10.035. Epub 2008 Oct 16.

Promoter-specific co-activation by Drosophila mastermind.

Author information

1
Department of Neuroscience, Weill Cornell Medical College, Burke Medical Research Institute, 785 Mamaroneck Ave, White Plains, New York, NY 10021, USA; Burke Medical Research Institute, White Plains, NY, 10605, USA. Electronic address: joc2042@med.cornell.edu.
2
Department of Neuroscience, Weill Cornell Medical College, Burke Medical Research Institute, 785 Mamaroneck Ave, White Plains, New York, NY 10021, USA; Burke Medical Research Institute, White Plains, NY, 10605, USA.

Abstract

Mastermind (Mam) is a co-activator protein of binary complexes consisting of Suppressor of Hairless (Su(H)) and Notch Intracellular Domain (NICD) proteins assembled on cis-regulatory regions of target genes activated by Notch signaling. Current evidence indicates that Mastermind is necessary and sufficient for the formation of a functional Su(H)/NICD/Mam ternary complex on at least one specific architecture of Su(H) binding sites, called the SPS element (Su(H) Paired Sites). However, using transcription assays with a combination of native and synthetic Notch target gene promoters in Drosophila cultured cells, we show here that co-activation of Su(H)/NICD complexes on SPS elements by Mam is promoter-specific. Our novel results suggest this promoter specificity is mediated by additional unknown cis-regulatory elements present in the native promoters that are required for the recruitment of Mam and formation of functional Su(H)/NICD/Mam complexes on SPS elements. Together, the findings in this study suggest Mam is not always necessary and sufficient for co-activation of binary Su(H)/NICD complexes on SPS elements.

PMID:
18930034
PMCID:
PMC2643046
DOI:
10.1016/j.bbrc.2008.10.035
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center