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Methods. 2008 Nov;46(3):177-82. doi: 10.1016/j.ymeth.2008.09.014. Epub 2008 Oct 16.

Studying isoform-specific inositol 1,4,5-trisphosphate receptor function and regulation.

Author information

1
Department of Pharmacology and Physiology, University of Rochester, 601 Elmwood Avenue, Rochester, NY 14642, USA.

Abstract

Inositol 1,4,5-trisphosphate receptors (InsP3R) are a family of ubiquitously expressed intracellular Ca2+ channels. Isoform-specific properties of the three family members may play a prominent role in defining the rich diversity of the spatial and temporal characteristics of intracellular Ca2+ signals. Studying the properties of the particular family members is complicated because individual receptor isoforms are typically never expressed in isolation. In this article, we discuss strategies for studying Ca2+ release through individual InsP3R family members with particular reference to methods applicable following expression of recombinant InsP3R and mutant constructs in the DT40-3KO cell line, an unambiguously null InsP3R expression system.

PMID:
18929664
PMCID:
PMC3069621
DOI:
10.1016/j.ymeth.2008.09.014
[Indexed for MEDLINE]
Free PMC Article

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