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Eur J Clin Chem Clin Biochem. 1991 May;29(5):327-30.

Isolation of restrictible DNA.

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  • 1Institute of Clinical Laboratory Diagnostics, Zagreb University School of Medicine, Yugoslavia.

Abstract

A simple method for the isolation of pure and high-yield DNA from whole blood, suitable for restriction enzyme digestion, is described. The steps of the procedure are as follows: cell lysis with NH4Cl, NaHCO3, EDTA; digestion with proteinase K in the presence of SDS; extraction with phenol-chloroform-isoamyl alcohol; and precipitation with ethanol. The 260 nm/280 nm absorbance ratio showed a mean value of 2, and the average yield of DNA was 212 micrograms/l. Such DNA preparations were found to be quite suitable for digestion by a variety of restriction endonucleases, as well as for the analysis of gene disorders by different biological methods. The method proposed appears to be useful in clinical chemistry laboratories.

PMID:
1892954
[PubMed - indexed for MEDLINE]
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