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Bioorg Med Chem Lett. 2008 Nov 15;18(22):5982-6. doi: 10.1016/j.bmcl.2008.09.083. Epub 2008 Sep 30.

Chemical probes for profiling fatty acid-associated proteins in living cells.

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The Laboratory of Chemical Biology and Microbial Pathogenesis, The Rockefeller University, 1230 York Avenue, Box 250, New York, NY 10065, USA.


Chemical probes appended with reactive electrophiles afford powerful tools for profiling discrete protein families in living cells. Herein, we have synthesized cell-permeable chemical probes that target fatty acid-associated proteins. These fatty acid-based chemical probes contain acyloxymethylketone or fluorophosphonate functional groups and an alkyne click chemistry tag for visualization of covalently modified proteins by in-gel fluorescence scanning. Our fatty acid-based chemical probe affords new tools to evaluate the activity/expression of lipid-associated proteins that should facilitate their functional characterization and inhibitor discovery.

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