Format

Send to

Choose Destination
See comment in PubMed Commons below
Clin Vaccine Immunol. 2008 Dec;15(12):1834-8. doi: 10.1128/CVI.00238-08. Epub 2008 Oct 15.

Multiplex immunoassay for serological diagnosis of Mycobacterium bovis infection in cattle.

Author information

1
Enfer Scientific, Unit T, M7 Business Park, Newhall, Naas, County Kildare, Ireland.

Abstract

Efforts to develop a better diagnostic assay for bovine tuberculosis have shown that the sensitivity and specificity of an assay can be improved by the use of two or more antigens. As reported here, we developed a multiplex chemiluminescent immunoassay that can simultaneously detect antibody activity to 25 antigens in a single well in a 96-well plate array format. The chemiluminescent signal is captured with a digital imaging system and analyzed with a macro program that tracks each serum for its pattern of antibody activity for Mycobacterium bovis antigens. The comparison of sera from 522 infected and 1,489 uninfected animals showed that a sensitivity of 93.1% and a specificity of 98.4% can be achieved with a combination of antigens. The assay system is rapid and can be automated for use in a centralized laboratory.

PMID:
18927068
PMCID:
PMC2593169
DOI:
10.1128/CVI.00238-08
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Support Center