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J Struct Biol. 2009 Jan;165(1):1-9. doi: 10.1016/j.jsb.2008.09.006. Epub 2008 Sep 30.

JADAS: a customizable automated data acquisition system and its application to ice-embedded single particles.

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1
Program in Structural and Computational Biology and Molecular Biophysics, Baylor College of Medicine, Houston, TX 77030, USA.

Abstract

The JEOL Automated Data Acquisition System (JADAS) is a software system built for the latest generation of the JEOL Transmission Electron Microscopes. It is designed to partially or fully automate image acquisition for ice-embedded single particles under low dose conditions. Its built-in flexibility permits users to customize the order of various imaging operations. In this paper, we describe how JADAS is used to accurately locate and image suitable specimen areas on a grid of ice-embedded particles. We also demonstrate the utility of JADAS by imaging the epsilon 15 bacteriophage with the JEM3200FSC electron cryo-microscope, showing that sufficient images can be collected in a single 8h session to yield a subnanometer resolution structure which agrees with the previously determined structure.

PMID:
18926912
PMCID:
PMC2634810
DOI:
10.1016/j.jsb.2008.09.006
[Indexed for MEDLINE]
Free PMC Article
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