Locally generated methylseleninic acid induces specific inactivation of protein kinase C isoenzymes: relevance to selenium-induced apoptosis in prostate cancer cells

J Biol Chem. 2008 Dec 12;283(50):34519-31. doi: 10.1074/jbc.M807007200. Epub 2008 Oct 15.

Abstract

In this study, we show that methylselenol, a selenometabolite implicated in cancer prevention, did not directly inactivate protein kinase C (PKC). Nonetheless, its oxidation product, methylseleninic acid (MSA), inactivated PKC at low micromolar concentrations through a redox modification of vicinal cysteine sulfhydryls in the catalytic domain of PKC. This modification of PKC that occurred in both isolated form and in intact cells was reversed by a reductase system involving thioredoxin reductase, a selenoprotein. PKC isoenzymes exhibited variable sensitivity to MSA with Ca(2+)-dependent PKC isoenzymes (alpha, beta, and gamma) being the most susceptible, followed by isoenzymes delta and epsilon. Other enzymes tested were inactivated only with severalfold higher concentrations of MSA than those required for PKC inactivation. This specificity for PKC was further enhanced when MSA was generated within close proximity to PKC through a reaction of methylselenol with PKC-bound lipid peroxides in the membrane. The MSA-methylselenol redox cycle resulted in the catalytic oxidation of sulfhydryls even with nanomolar concentrations of selenium. MSA inhibited cell growth and induced apoptosis in DU145 prostate cancer cells at a concentration that was higher than that needed to inhibit purified PKC alpha but in a range comparable with that required for the inhibition of PKC epsilon. This MSA-induced growth inhibition and apoptosis decreased with a conditional overexpression of PKC epsilon and increased with its knock-out by small interfering RNA. Conceivably, when MSA is generated within the vicinity of PKC, it specifically inactivates PKC isoenzymes, particularly the promitogenic and prosurvival epsilon isoenzyme, and this inactivation causes growth inhibition and apoptosis.

MeSH terms

  • Apoptosis*
  • Catalytic Domain
  • Cell Line, Tumor
  • Cell Proliferation
  • Cell Survival / drug effects
  • Drug Resistance, Neoplasm
  • Humans
  • Isoenzymes / chemistry
  • Male
  • Organoselenium Compounds / metabolism*
  • Oxygen / chemistry
  • Prostatic Neoplasms / enzymology*
  • Prostatic Neoplasms / metabolism
  • Protein Kinase C / chemistry
  • Protein Kinase C / metabolism*
  • RNA, Small Interfering / metabolism
  • Selenium / pharmacology*

Substances

  • Isoenzymes
  • Organoselenium Compounds
  • RNA, Small Interfering
  • methylselenic acid
  • Protein Kinase C
  • Selenium
  • Oxygen