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PLoS Biol. 2008 Oct 14;6(10):e252. doi: 10.1371/journal.pbio.0060252.

The nucleosome-remodeling ATPase ISWI is regulated by poly-ADP-ribosylation.

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1
Istituto Telethon Dulbecco, Universita' degli Studi di Palermo, Palermo, Italy.

Abstract

ATP-dependent nucleosome-remodeling enzymes and covalent modifiers of chromatin set the functional state of chromatin. However, how these enzymatic activities are coordinated in the nucleus is largely unknown. We found that the evolutionary conserved nucleosome-remodeling ATPase ISWI and the poly-ADP-ribose polymerase PARP genetically interact. We present evidence showing that ISWI is target of poly-ADP-ribosylation. Poly-ADP-ribosylation counteracts ISWI function in vitro and in vivo. Our work suggests that ISWI is a physiological target of PARP and that poly-ADP-ribosylation can be a new, important post-translational modification regulating the activity of ATP-dependent nucleosome remodelers.

PMID:
18922045
PMCID:
PMC2567001
DOI:
10.1371/journal.pbio.0060252
[Indexed for MEDLINE]
Free PMC Article
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