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Anal Biochem. 1991 Jul;196(1):61-8.

Detection apparatus for multiple heterogeneous chemiluminescence immunoassay configurations.

Author information

1
Diagnostics Division, Abbott Laboratories, Abbott Park, Illinois 60064.

Abstract

We describe an apparatus for measuring signals emanated from two heterogeneous chemiluminescence immunoassay (CLIA) configurations: antibody-coated polystyrene beads, in reaction tray wells, and microparticles captured by a porous matrix. An optics and fluidics design which allows the use of a common detection head for these two different assay configurations is described. The detection head moves along three Cartesian coordinates to create a localized light-tight compartment around each individual disposable reaction vessel. Reproducibility of the light seal, trigger solution delivery, and mixing is achieved for acridinium-labeled CLIA. The coated polystyrene beads configuration is tested using beta HCG, CEA, and TSH assays. The microparticle-capture configuration is tested using beta HCG and HBsAg assays. The microparticle capture CLIA has shorter incubation times and the potential for ease of automation.

PMID:
1888037
DOI:
10.1016/0003-2697(91)90117-c
[Indexed for MEDLINE]

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