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J Med Virol. 1991 May;34(1):51-4.

Demonstration of concurrent dengue 1 and dengue 3 infection in six patients by the polymerase chain reaction.

Author information

1
Laboratoire des Arbovirus, Institut Pasteur de Nouvelle Calédonie, Nouméa, New Caledonia.

Abstract

A dual viremia resulting from naturally acquired dengue 1 and dengue 3 infections in six patients experiencing a dengue-like syndrome during the epidemic in New Caledonia in 1989 is reported. Serotype identification was first based on virus isolation in mosquito cells and immunofluorescence using type-specific monoclonal antibodies. The double infection was confirmed directly in blood samples by the polymerase chain reaction (PCR) on genomic RNA and hybridization of the amplified cDNA fragments with type-specific DNA probes.

PMID:
1885943
DOI:
10.1002/jmv.1890340109
[Indexed for MEDLINE]

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