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J Histochem Cytochem. 2009 Jan;57(1):1-5. doi: 10.1369/jhc.2008.952770. Epub 2008 Oct 14.

A guide to the perplexed on the specificity of antibodies.

Author information

1
Department of Neurology, Beth Israel Deaconess Medical Center, 330 Brookline Avenue, Boston, MA 02215, USA. csaper@bidmc.harvard.edu

Abstract

Many investigators are unaware of the potential problems with specificity of antibodies and the need to document antibody characterization meticulously for each antibody that is used. In this review, I consider the principles of antibody action and how they define a set of rules for what information should be obtained by the investigator before using an antibody in a serious scientific investigation.

PMID:
18854594
PMCID:
PMC2605712
DOI:
10.1369/jhc.2008.952770
[Indexed for MEDLINE]
Free PMC Article
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