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FEMS Microbiol Lett. 1991 Jun 15;65(2):151-5.

The role of the serine protease active site in the mode of action of epidermolytic toxin of Staphylococcus aureus.

Author information

1
Department of Biochemistry, Trinity College, Dublin, Republic of Ireland.

Abstract

The sequences of the epidermolytic toxins and V8 serine proteinase share about 25% identity, including the catalytic triad at the proteinase active centre. Here we have altered the putative ETA active-site serine-195 to glycine by site-directed mutagenesis. No epidermolytic activity was detected when up to 100-fold greater amounts of the homogeneous mutant ETA were injected subcutaneously into neonatal mice showing that serine-195 is required for toxicogenesis.

PMID:
1884990
DOI:
10.1016/0378-1097(91)90295-l
[Indexed for MEDLINE]

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