IgE-induced degranulation of mucosal mast cells is negatively regulated via nicotinic acetylcholine receptors

Natsuko Kageyama-Yahara; Yoko Suehiro; Takeshi Yamamoto; Makoto Kadowaki

doi:10.1016/j.bbrc.2008.10.004

IgE-induced degranulation of mucosal mast cells is negatively regulated via nicotinic acetylcholine receptors

Biochem Biophys Res Commun. 2008 Dec 5;377(1):321-5. doi: 10.1016/j.bbrc.2008.10.004. Epub 2008 Oct 10.

Authors

Natsuko Kageyama-Yahara¹, Yoko Suehiro, Takeshi Yamamoto, Makoto Kadowaki

Affiliation

¹ Department of Bioscience, Division of Gastrointestinal Pathophysiology, Institute of Natural Medicine, University of Toyama, Sugitani, Toyama, Japan. natsuko@inm.u-toyama.ac.jp

PMID: 18848921
DOI: 10.1016/j.bbrc.2008.10.004

Abstract

The autonomic nervous system is known to mediate mast cell activation. We investigated expression of nicotinic acetylcholine receptors (nAChRs) in mucosal-type mast cells and their contribution to the regulation of mast cell activation. Expression of mRNA of nAChR alpha4, alpha7, and beta2 subunits were detected in specially differentiated mucosal-type murine bone marrow-derived mast cells (mBMMCs). Pretreatment with non-specific nAChRs agonists, acetylcholine, nicotine and epibatidine and a specific alpha7 subunit agonist GTS-21 significantly inhibited antigen-induced degranulation of mBMMCs in a dose-dependent manner and GTS-21-induced inhibition was significantly blocked by alpha7 subunit antagonist, alpha-bungarotoxin. Furthermore, confocal microscopy also demonstrated surface binding of alpha-bungarotoxin on mBMMCs. Our findings indicate that mucosal mast cell activation may be negatively regulated mainly through nAChR alpha7 subunit, suggesting that nAChRs are involved in neuronal-mucosal mast cell interactions.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Benzylidene Compounds / pharmacology
Bone Marrow Cells / metabolism
Bone Marrow Cells / physiology
Bungarotoxins / chemistry
Bungarotoxins / pharmacology
Cell Degranulation*
Cell Membrane / chemistry
Cell Membrane / metabolism
Fluorescein-5-isothiocyanate / chemistry
Immunoglobulin E / immunology
Immunoglobulin E / pharmacology
Intestinal Mucosa / metabolism
Intestinal Mucosa / physiology*
Mast Cells / metabolism
Mast Cells / physiology*
Mice
Nicotinic Agonists / pharmacology
Pyridines / pharmacology
RNA, Messenger / metabolism
Receptors, Nicotinic / drug effects
Receptors, Nicotinic / genetics
Receptors, Nicotinic / metabolism*
alpha7 Nicotinic Acetylcholine Receptor

Substances

Benzylidene Compounds
Bungarotoxins
Chrna7 protein, mouse
Nicotinic Agonists
Pyridines
RNA, Messenger
Receptors, Nicotinic
alpha7 Nicotinic Acetylcholine Receptor
nicotinic acetylcholine receptor alpha4 subunit
nicotinic receptor beta2
Immunoglobulin E
3-(2,4-dimethoxybenzylidene)anabaseine
Fluorescein-5-isothiocyanate