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J Clin Invest. 2008 Nov;118(11):3790-7. doi: 10.1172/JCI36209. Epub 2008 Oct 9.

Real-time, multidimensional in vivo imaging used to investigate blood flow in mouse pancreatic islets.

Author information

1
Department of Medicine, Vanderbilt University, Nashville, Tennessee, USA.

Abstract

The pancreatic islets of Langerhans are highly vascularized micro-organs that play a key role in the regulation of blood glucose homeostasis. The specific arrangement of endocrine cell types in islets suggests a coupling between morphology and function within the islet. Here, we established a line-scanning confocal microscopy approach to examine the relationship between blood flow and islet cell type arrangement by real-time in vivo imaging of intra-islet blood flow in mice. These data were used to reconstruct the in vivo 3D architecture of the islet and time-resolved blood flow patterns throughout the islet vascular bed. The results revealed 2 predominant blood flow patterns in mouse islets: inner-to-outer, in which blood perfuses the core of beta cells before the islet perimeter of non-beta cells, and top-to-bottom, in which blood perfuses the islet from one side to the other regardless of cell type. Our approach included both millisecond temporal resolution and submicron spatial resolution, allowing for real-time imaging of islet blood flow within the living mouse, which has not to our knowledge been attainable by other methods.

PMID:
18846254
PMCID:
PMC2564611
DOI:
10.1172/JCI36209
[Indexed for MEDLINE]
Free PMC Article

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