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Org Lett. 2008 Nov 6;10(21):4931-4. doi: 10.1021/ol801907b. Epub 2008 Oct 9.

Nonclassical SNAPFL analogue as a Cy5 resonance energy transfer partner.

Author information

1
Department of Chemistry of University of Illinois, 600 South Mathews Avenue, Urbana, Illinois 61801, USA.

Abstract

We have synthesized a new SNAPFL analogue (1) that exhibits a large Stokes shift (78 nm) (abs. 542 nm, em. 620 nm) and a good quantum yield. Because of the large overlap between the emission spectrum of 1 and the absorption spectrum of Cy5, 1 functions well as a fluorescence donor to Cy5 and has been used in FRET-based experiments using estrogen receptor site-specifically labeled with Cy5 and a receptor ligand conjugated to SNAPFL.

PMID:
18841988
PMCID:
PMC2787200
DOI:
10.1021/ol801907b
[Indexed for MEDLINE]
Free PMC Article

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