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FEBS Lett. 2008 Oct 29;582(25-26):3643-9. doi: 10.1016/j.febslet.2008.09.047. Epub 2008 Oct 7.

Interaction of fatty acid with myoglobin.

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1
Department of Biochemistry and Molecular Medicine, University of California Davis, Davis, CA 95616-8635, USA.

Abstract

Upon titration with palmitate, the (1)H NMR spectra of metmyoglobin cyanide (MbCN) reveal a selective perturbation of the 8 heme methyl, consistent with a specific interaction of myoglobin (Mb) with fatty acid. Other detectable hyperfine shifted resonances of the heme group remain unchanged. Mb also enhances fatty acid solubility, as reflected in a more intense methylene peak of palmitate in Mb solution than in Tris buffer. Ligand binding analysis indicates an apparent palmitate dissociation constant (K(d)) of 43microM. These results suggest that Mb can bind fatty acid and may have a role in facilitating fatty acid transport in the cell.

PMID:
18840435
PMCID:
PMC2591068
DOI:
10.1016/j.febslet.2008.09.047
[Indexed for MEDLINE]
Free PMC Article
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