Format

Send to

Choose Destination
Methods Mol Biol. 2009;493:241-55. doi: 10.1007/978-1-59745-523-7_14.

Twist-off purification of hair bundles.

Author information

1
Oregon Hearing Research Center and Vollum Institute, Oregon Health & Science University, Portland, OR, USA.

Abstract

Purification of hair bundles from inner-ear organs allows biochemical analysis of bundle constituents, including proteins and lipids. We describe here the "twist-off" method of bundle isolation, where dissected inner-ear organs are embedded in agarose, then subjected to a mechanical disruption that shears off bundles and leaves them in agarose blocks. With care in the dissection and in clean-up of the isolated bundles, contamination from cell bodies can be kept to a minimum. Isolated bundles can be analyzed by a variety of techniques, including immunocytochemistry, SDS-PAGE, immunoblotting, and mass spectrometry.

PMID:
18839351
DOI:
10.1007/978-1-59745-523-7_14
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center