Format

Send to

Choose Destination
BMC Immunol. 2008 Sep 29;9:55. doi: 10.1186/1471-2172-9-55.

Host immunity in the protective response to vaccination with heat-killed Burkholderia mallei.

Author information

1
Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas, USA. gcwhilo@utmb.edu

Abstract

BACKGROUND:

We performed initial cell, cytokine and complement depletion studies to investigate the possible role of these effectors in response to vaccination with heat-killed Burkholderia mallei in a susceptible BALB/c mouse model of infection.

RESULTS:

While protection with heat-killed bacilli did not result in sterilizing immunity, limited protection was afforded against an otherwise lethal infection and provided insight into potential host protective mechanisms. Our results demonstrated that mice depleted of either B cells, TNF-alpha or IFN-gamma exhibited decreased survival rates, indicating a role for these effectors in obtaining partial protection from a lethal challenge by the intraperitoneal route. Additionally, complement depletion had no effect on immunoglobulin production when compared to non-complement depleted controls infected intranasally.

CONCLUSION:

The data provide a basis for future studies of protection via vaccination using either subunit or whole-organism vaccine preparations from lethal infection in the experimental BALB/c mouse model. The results of this study demonstrate participation of B220+ cells and pro-inflammatory cytokines IFN-gamma and TNF-alpha in protection following HK vaccination.

PMID:
18823549
PMCID:
PMC2562362
DOI:
10.1186/1471-2172-9-55
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for BioMed Central Icon for PubMed Central
Loading ...
Support Center