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Nanomedicine (Lond). 2008 Oct;3(5):647-67. doi: 10.2217/17435889.3.5.647.

LANTCET: elimination of solid tumor cells with photothermal bubbles generated around clusters of gold nanoparticles.

Author information

1
A.V. Lykov Heat & Mass Transfer Institute, 15 P. Brovka Street, Minsk, 220072, Belarus.

Abstract

BACKGROUND:

We have developed a method, termed laser-activated nano-thermolysis as a cell elimination technology (LANTCET), for the selective detection and destruction of individual tumor cells by the generation of intracellular photothermal bubbles around clusters of gold nanoparticles.

METHOD:

Bare nanoparticles and their conjugates to C225 tumor-specific monoclonal antibodies were applied in vitro to C225-positive squamous carcinoma cells and in vivo to an experimental tumor in a rat in order to form intracellular clusters of nanoparticles.

RESULTS:

Single 10 ns laser pulses generated intracellular photothermal microbubbles at a near-infrared and visible wavelengths. The cells with the clusters yielded an almost 100-fold decrease in the laser fluence threshold for bubble generation and cell damage relative to that for the cells without clusters. Cell damage had a mechanical origin and single cell selectivity. Three LANTCET processes (cell detection, damage and optical guidance) were realized as a microsecond sequence and with the one device.

PMID:
18817468
DOI:
10.2217/17435889.3.5.647
[Indexed for MEDLINE]
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