Format

Send to

Choose Destination
J Proteome Res. 2008 Nov;7(11):4715-26. doi: 10.1021/pr800255a. Epub 2008 Sep 25.

Screening for EphB signaling effectors using SILAC with a linear ion trap-orbitrap mass spectrometer.

Author information

1
Department of Pharmacology and Kimmel Center for Biology and Medicine at the Skirball Institute, New York University School of Medicine, New York, New York 10016, USA.

Abstract

Erythropoietin-producing hepatocellular carcinoma (Eph) receptors play important roles in development, neural plasticity, and cancer. We used an Orbitrap mass spectrometer and stable isotope labeling by amino acids in cell culture (SILAC) to identify and quantify 204 proteins with significantly changed abundance in antiphosphotyrosine immunoprecipitates after ephrinB1-Fc stimulation. More than half of all known effectors downstream of EphB receptors were identified in this study, as well as numerous novel candidates for EphB signaling.

PMID:
18816084
PMCID:
PMC2673988
DOI:
10.1021/pr800255a
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for American Chemical Society Icon for PubMed Central
Loading ...
Support Center