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Epidemiology. 2009 Jan;20(1):36-43. doi: 10.1097/EDE.0b013e318187865e.

Intraindividual variability in serum micronutrients: effects on reliability of estimated parameters.

Author information

1
Cancer Research Center of Hawaii, University of Hawaii, Honolulu, HI 96813, USA. yshvetso@crch.hawaii.edu

Abstract

BACKGROUND:

Although serum measures of micronutrients are more specific than questionnaires in quantifying nutritional status, the reliability of serum measures depends on between- and within-person variability of circulating micronutrient levels. The extent to which multiple samples per person might improve reliability is useful information for planning studies and interpreting results.

METHODS:

We analyzed levels of 25 micronutrients in serum samples taken from 381 Hawaii women at 4-month intervals. For all subjects and for subjects at the low and high end of the micronutrient distributions, we calculated inter- and intraindividual variability, reliability coefficients, and the number of measurements required to limit attenuation in estimated parameters (ie, to keep estimates close to their true values).

RESULTS:

For 18 of the 25 micronutrients, a single measurement provided an estimate within 20% of the true value. For regression coefficients, 2 measurements were needed to limit attenuation to no more than 20% for nearly half of the micronutrients. To achieve no more than 10% attenuation, the number of measurements required ranged from 2 to 10 for correlation and from 3 to 20 for regression coefficients. To achieve no more than 5% attenuation, the corresponding ranges were 3 to 21 for correlation and 6 to 42 regression coefficients. In general, more measurements were required for adequate characterization of subjects with relatively high levels of micronutrients than for subjects with lower levels.

CONCLUSIONS:

Our analysis suggests that 2 or 3 blood measurements are enough to limit attenuation of regression coefficients within 20% of the true value for most carotenoids and tocopherols. For 10% attenuation or less, 4 or more micronutrient measurements may be needed.

PMID:
18813024
PMCID:
PMC2663917
DOI:
10.1097/EDE.0b013e318187865e
[Indexed for MEDLINE]
Free PMC Article

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