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Biochem Biophys Res Commun. 2008 Dec 5;377(1):23-8. doi: 10.1016/j.bbrc.2008.09.055. Epub 2008 Sep 20.

Crystal structure of the Bruton's tyrosine kinase PH domain with phosphatidylinositol.

Author information

1
Graduate School of Biomedical Engineering, Tohoku University, Sendai, Japan.

Abstract

Bruton's tyrosine kinase (Btk) of the Tec family possesses a Pleckstrin homology (PH) domain, which is responsible for plasma membrane targeting. In this study, the crystal structure of the Btk PH domain in complex with dibutylyl-phosphatidylinositol-3,4,5-triphosphate was determined. The structure revealed that the Btk PH domain forms a homodimer and that each molecule binds phosphatidylinositol in the binding pocket. The side chain of Lys18 within a Btk-specific insertion in the beta1-beta2 loop is able to form a hydrogen bond with the diacylglycerol moiety of phosphatidylinositol. The other Btk-specific insertion in the beta5-beta6 loop constitutes the dimerization interface. Thus, the modes of phosphatidylinositol recognition and Btk PH domain dimerization are distinct from those of other PH domains.

PMID:
18809383
DOI:
10.1016/j.bbrc.2008.09.055
[Indexed for MEDLINE]

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