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Neural Dev. 2008 Sep 22;3:23. doi: 10.1186/1749-8104-3-23.

A chemical-genetic strategy reveals distinct temporal requirements for SAD-1 kinase in neuronal polarization and synapse formation.

Author information

1
Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada. joanne.kim@utoronto.ca

Abstract

BACKGROUND:

Neurons assemble into a functional network through a sequence of developmental processes including neuronal polarization and synapse formation. In Caenorhabditis elegans, the serine/threonine SAD-1 kinase is essential for proper neuronal polarity and synaptic organization. To determine if SAD-1 activity regulates the establishment or maintenance of these neuronal structures, we examined its temporal requirements using a chemical-genetic method that allows for selective and reversible inactivation of its kinase activity in vivo.

RESULTS:

We generated a PP1 analog-sensitive variant of SAD-1. Through temporal inhibition of SAD-1 kinase activity we show that its activity is required for the establishment of both neuronal polarity and synaptic organization. However, while SAD-1 activity is needed strictly when neurons are polarizing, the temporal requirement for SAD-1 is less stringent in synaptic organization, which can also be re-established during maintenance.

CONCLUSION:

This study reports the first temporal analysis of a neural kinase activity using the chemical-genetic system. It reveals that neuronal polarity and synaptic organization have distinct temporal requirements for SAD-1.

PMID:
18808695
PMCID:
PMC2564922
DOI:
10.1186/1749-8104-3-23
[Indexed for MEDLINE]
Free PMC Article

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