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Virology. 2008 Nov 10;381(1):67-74. doi: 10.1016/j.virol.2008.08.021. Epub 2008 Sep 17.

Temperature-dependent production of pseudoinfectious dengue reporter virus particles by complementation.

Author information

1
Viral Pathogenesis Section, Laboratory of Viral Diseases, National Institutes of Health, Bethesda, MD, USA.

Abstract

Dengue virus (DENV) is a mosquito-borne flavivirus responsible for 50 to 100 million human infections each year, highlighting the need for a safe and effective vaccine. In this study, we describe the production of pseudoinfectious DENV reporter virus particles (RVPs) using two different genetic complementation approaches, including the creation of cell lines that release reporter viruses in an inducible fashion. In contrast to studies with West Nile virus (WNV), production of infectious DENV RVPs was temperature-dependent; the yield of infectious DENV RVPs at 37 degrees C is significantly reduced in comparison to experiments conducted at lower temperatures or with WNV. This reflects both a significant reduction in the rate of infectious DENV RVP release over time, and the more rapid decay of infectious DENV RVPs at 37 degrees C. Optimized production approaches allow the production of DENV RVPs with titers suitable for the study of DENV entry, assembly, and the analysis of the humoral immune response of infected and vaccinated individuals.

PMID:
18801552
PMCID:
PMC3428711
DOI:
10.1016/j.virol.2008.08.021
[Indexed for MEDLINE]
Free PMC Article

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