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J Antimicrob Chemother. 2008 Dec;62(6):1199-202. doi: 10.1093/jac/dkn387. Epub 2008 Sep 16.

Mycobacterium tuberculosis DNA repair in response to subinhibitory concentrations of ciprofloxacin.

Author information

1
Centre for Medical Microbiology, Department of Infection, Royal Free Campus, University College London, Rowland Hill Street, Hampstead, London NW3 2PF, UK.

Abstract

OBJECTIVES:

To investigate how the SOS response, an error-prone DNA repair pathway, is expressed following subinhibitory quinolone treatment of Mycobacterium tuberculosis.

METHODS:

Genome-wide expression profiling followed by quantitative RT (qRT)-PCR was used to study the effect of ciprofloxacin on M. tuberculosis gene expression.

RESULTS:

Microarray analysis showed that 16/110 genes involved in DNA protection, repair and recombination were up-regulated. There appeared to be a lack of downstream genes involved in the SOS response. qRT-PCR detected an induction of lexA and recA after 4 h and of dnaE2 after 24 h of subinhibitory treatment.

CONCLUSIONS:

The pattern of gene expression observed following subinhibitory quinolone treatment differed from that induced after other DNA-damaging agents (e.g. mitomycin C). The expression of the DnaE2 polymerase response was significantly delayed following subinhibitory quinolone exposure.

PMID:
18799471
PMCID:
PMC2583065
DOI:
10.1093/jac/dkn387
[Indexed for MEDLINE]
Free PMC Article

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