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J Basic Microbiol. 2009 Apr;49(2):216-9. doi: 10.1002/jobm.200800167.

Isolation and characterization of phenol degrading yeast.

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Department of Biochemistry and Biotechnology, Institute of Science, Nirma University of Science and Technology, Sarkhej-Gandhinagar Highway, Ahmedabad Gujarat, India.


A phenol degrading yeast isolate was identified and characterized from the soil sample collected from a landfill site, in Ahmedabad, India, by plating the soil dilutions on Sabouraud's Dextrose Agar. The microscopic studies and biochemical tests indicated the isolate to be Saccharomyces cerevisiae. The phenol degrading potential of the isolate was measured by inoculation of pure culture in the mineral medium containing various phenol concentrations ranging from 100 to 800 mg l(-1 )and monitoring phenol disappearance rate at regular intervals of time. Growth of the isolate in mineral medium with various phenol concentrations was monitored by measuring the turbidity (OD(600) nm). The results showed that the isolated yeast was tolerant to phenol up to 800 mg(-1). The phenol degradation ranged from 8.57 to 100% for the concentration of phenol from 800 mg l(-1 )to 200 mg l(-1), respectively.

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